Deoxyribose Aldolase from Lactobacihs plantarum
نویسندگان
چکیده
The fermentation of deoxyribose in Escherichia coli (1, 2) and in Lactobacillus plantarum (3, 4) appears to involve 2-deoxyribose 5-phosphate as an intermediate. Racker (2) has described an enzyme, deoxyribose phosphate aldolase, which catal.yzes the reversible cleavage of deoxyribose 5-phosphate to acetaldehyde and glyceraldehyde 3-phosphate. The enzyme occurs in E. coli and in animal tissues (2), where its function is not clearly established, and in deoxyribose-grown cells of L. plantarum, where it plays a major role in the fermentation pathway (3). The present paper is a report of studies on the induction, purification, and kinetic properties of deoxyribose phosphate aldolase of L. plantarum, together with a method for the synthesis and isolation of 2-deoxyribose 5-phosphate.
منابع مشابه
Deoxyribose Phosphate Aldolase from Rat Liver.
The activity and properties of deoxyribose phosphate aldolase from Escherichia coli and Lactobacillus plantarum have been described in detail by Racker (1) and Pricer and Horecker (2). The enzyme from E. coli was shown to be active at pH values between 6.0 and 8.0 in tris(hydroxymethyl)aminomethane and phosphate buffers without exhibiting a sharp optimum (1). The enzyme from L. plunturum was sh...
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